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PUC19 Vector Cloning

This post categorized under Vector and posted on July 4th, 2019.

Puc19 is a commonly used cloning vector that conveys Tet and Amp resistance. The molecule is a small double-stranded circle 2686 base pairs in vectorgth and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).pUC19 Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. The MCS is reversed in pUC18.pUC19 Vector Information Description. pUC19 is a small high-copy number E. coli plasmid cloning vector of which multiple cloning sites as shown below.

Vector pUC1819 and pUC118119. The plasmids have a multiple cloning site within the lacZ alpha-fragment. Inserts cloned into this site disrupt beta -galactosidase activity and give rise to white colonies on X-GalIPTG plates.Thermo Scientific pUC19 vector is a small high copy number E. coli plasmid 2686 bp in vectorgth. It contains identical multiple cloning site (MCS) as pUC18 vector except that Plasmid pUC19 from Dr. Joachim Messings lab is published in Gene. 1983 Dec26(1)101-6. This plasmid is available through Addgene.

PUC19 gehrt wie auch pUC18 zu einer Reihe im Labor von Joachim Messing gentechnologisch hergestellter Klonierungsvektoren. Diese bakterielvector Plasmide gehren zu den am hufigsten verwendeten Vektoren zur Klonierung im Bakterium Escherichia coli .

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